Multiprotein machines for gene transcription
نویسندگان
چکیده
منابع مشابه
A multiprotein complex necessary for both transcription and DNA replication at the β-globin locus.
DNA replication, repair, transcription and chromatin structure are intricately associated nuclear processes, but the molecular links between these events are often obscure. In this study, we have surveyed the protein complexes that bind at β-globin locus control region, and purified and characterized the function of one such multiprotein complex from human erythroleukemic K562 cells. We further...
متن کاملA multiprotein complex necessary for both transcription and DNA replication at the beta globin locus
The referees find that the biochemistry identifying DAART to be relatively convincing, referee #1 would like to see a sucrose gradient to determine if a single complex or several sub complexes exist. However, both referees raise concerns with the data showing that the complex plays a role in replication origin activation and beta-globin transcription. In addition to several technical concerns t...
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Complementation for virulence of a non-polar virB5 mutant in Brucella suis 1330 was not possible using a pBBR-based plasmid but was with low copy vector pGL10. Presence of the pBBR-based replicon in wildtype B. suis had a dominant negative effect, leading to complete attenuation in J774 macrophages. This was due to pleiotropic effects on VirB protein expression due to multiple copies of the vir...
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DNA looping plays a fundamental role in a wide variety of biological processes, providing the backbone for long range interactions on DNA. Here we develop the first model for DNA looping by an arbitrarily large number of proteins and solve it analytically in the case of identical binding. We uncover a switchlike transition between looped and unlooped phases and identify the key parameters that ...
متن کاملThe APP intracellular domain forms nuclear multiprotein complexes and regulates the transcription of its own precursor.
The physiological functions of the beta-amyloid precursor protein (APP) may include nuclear signaling. To characterize the role of the APP adaptor proteins Fe65, Jip1b, X11alpha (MINT1) and the chromatin-associated protein Tip60, we analyzed their interactions by confocal microscopy and co-immunoprecipitations. AICD corresponding to S3-cleaved APP bound to Fe65 that transported it to nuclei and...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2007
ISSN: 0108-7673
DOI: 10.1107/s0108767307099837